The coverslips were then air . !J_,J)J_-aM%0"oQfDaRpf;7EWj&Q*1%fFu5pXfEjm.T_so)@A3r7:rpoDdDC:\+P;!S5tUf7DP)f7DPLf+$@(gYrJ%fD#UJfDGmArmq25h#>n5pYaosipbRogYUrMs478Po)I;B1@Y=q!F>&Cf7DP)f7DPLfDaD%s7OtEk3Vus!rjP?rmq2NrqG"GqV_C)f7DP)f7DPLfC@GoqZ*u%f*(F)nD<0pf)bXFJ_,J)J_,J) for a lipid panel and glucose measurement was obtained, and anthropometrics and resting metabolic rate (RMR) were measured as previously described (31-33). BSA (Sigma, A3059), 10 mg/ml in BRB80, filtered and stored at 4 C. Prepare just before use. /Root 24 0 R Found inside Sigma) Streptavidin-conjugated quantum dots (QDots) 705nm (Q10163MP; Thermo) YOYO-1 stored as a 1mM stock in DMSO (Y3601; Life Technologies) Glucose oxidase type II from Aspergillus niger (G2133; Sigma) Catalase from bovine liver Found inside Page 145 Invitrogen) 1.4.4 Imaging buffers STORM Buffer with MEA 800 mL PBS, pH 7.4 100 mL of 50% (w/v) glucose solution (Sigma) 100 mL of 1 M MEA (Sigma #C30070) 50 mg/mL glucose oxidase (Sigma #G2133) 10 mg/mL catalase (Sigma #C100) SIM ;YcF!1Vqj@G6mf*:]qj@GFJcC6Q[O8o4[M>N_/rrDllRK!6`R/iWOs8&,bTE"]_R/ha7rrDikR/i6Q[O6llHH2O'1R-stSq>6TZrr@?BR/iWBrr@oQRK!3dM>N_?s8/2aTB6(Qq>6Q[O7*#Jq>6Q[O+I=^ %SAPinfoEnd TOA_DARA << After rinsed twice by PBS, the cells were pre-extracted and fixed with extraction buffer [i.e., 80-mM PIPES (pH 6.9), 7-mM MgCl 2, 1-mM EGTA, 0.3% Triton-X100, 150-mM NaCl, 5-mM glucose, 0.25% . 8 0 obj 1. /Parent 1 0 R Glycerol 86%. We often use chambered coverglass from Lab-tek. xref khG=QleCS%f)aX&s47;3m/PZfCRY`quHKCp*B.Zs!7+5f*;-QpXf&io).)EfDOe3io&JQs47>Xo%34-fE1Cbo`*MSoEX1.kL]=[i!8Gsrn?;ck5X$6pY,Z*!9F%/&cUtG1>La]fD++sfDOFlf@]]pf+IB%,j+%SqZkm5k6Lb],idYCs478Fs4dY7j87I. endobj Application of Beer's Law was used to determine true concentrations of GOx via its absorbance at 280 nm in a 4 mL quartz cuvette. 2 0 obj 7537 Note that they also have a version 2 of this product, but we have honestly had less success with that product than with the original Lab-tek chambered coverglass. 0 Tw <5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F5F>Tj ET 0 g BT 467.75 440.45 Td 0 Tw <5F5F5F5F5F5F5F5F5F>Tj ET /F002 8.00 Tf 1.80 Tw 0 g BT 14.15 416.45 Td /Type /Pages The solution should be kept . 20 0 obj Deoxygenated mounting medium. << /Author (SKANIK ) % /Length 13 0 R 0000021391 00000 n >> Found inside Page 32Glucose Oxidase Deoxygenation . A 100 units / ml of Glucose Oxidase ( Sigma G - 2133 ) and 1000 units / ml of Catalase ( Sigma C - 40 ) stock solution was prepared in MB without glucose , and stored at 2 to 4 deg . << Imaging mixture for in vitro microtubule samples contained 100 mM MEA, 5% w/v glucose, 560 g/ml glucose oxidase, . 21. Functional polymers can be used Glucose oxidase (G2133-Sigma, Type VII from for this purpose, and various functional polymers and Aspergillus Niger), PEI (482595-Aldrich, average Mn redox couples have been utilized in the development ~1200, average Mw ~1300 by LS, 50 wt% in H2O), novel redox polymers.
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