Store the aliquots in a -20 C freezer. <3330353020537072756365205374726565742C205361696E74204C6F7569732C204D4F2036333130332C20555341>Tj ET 0 g BT 423.70 688.90 Td <576562736974653A20207777772E7369676D61616C64726963682E636F6D>Tj ET 0 g BT 415.00 676.90 Td
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Glucose Oxidase from Aspergillus niger . >>
19. U/ml glucose oxidase (Sigma # G2133), 2170 U/ml catalase (Roche # 10106810001), and 1 mM Trolox (Sigma) (2)). /Type /Font
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Found insideGlucose oxidase (Sigma: G2133): make up a 100 U/ml solution in PBS-Ca-Mg 5. Glucose 25 mg/ml in PBS-Ca-Mg 6. Na 125I carrier-free (Amersham No. IMS30) specific radioactivity (around 15 mCi/g) 3. Method For 2 107 cells, wash the cells 0000001548 00000 n
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Each subunit contains one flavin adenine dinulceotide moiety and one iron. s+13$s2=rsrr@o9R/i6Q[O7E5Oq#?-HR/ilZrrDllRIC.VO88[@!.Y%K! 50X Gloxy buffer was made by making 8440 AU glucose oxidase (Sigma #G2133, 50 KU) and 70200 AU catalase (Sigma C40, 100 mg) in 50 mM Tris (pH 8.0 . <417070656172616E63652028436F6C6F7229202020202020202020202020>Tj ET 0 g BT 316.55 416.45 Td <59656C6C6F77>Tj ET 0 g BT 14.15 404.45 Td <417070656172616E63652028466F726D2920202020202020202020202020>Tj ET 0 g BT 316.55 404.45 Td <506F77646572>Tj ET 0 g BT
Cell cycle is the process by which a cell divides into two daughter cells. 96-well Plate sealing sheets. Make a bunch of aliquots and store at -20C. /Flags 32
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Glucose Oxidase (e.g. >>
Found inside Page 6Hydrofluoric acid 40% Acetone 99% Ethanol 99.9% Hydrogen peroxide 35.5% Ammonium hydroxide 25% Hydrochloric acid 37% Alconox (powder) Tris 99.9% KCl 99.5% Glucose oxidase (GO), e.g. (Sigma G2133) Catalase (C), e.g. (Sigma C3155-50 mG) 0000000017 00000 n
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Make a bunch of aliquots and store at -20C. After rinsed twice by PBS, the cells were pre-extracted and fixed with extraction buffer [i.e., 80-mM PIPES (pH 6.9), 7-mM MgCl 2, 1-mM EGTA, 0.3% Triton-X100, 150-mM NaCl, 5-mM glucose, 0.25% . The book begins by describing the fundamentals of absorption, metabolism and bioavailability of polyphenols, as well as the effect of microbes on polyphenol structure and function and toxicity. 37090
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Glucose oxidase (GOx) (from Aspergillus niger, Sigma-Aldrich, G2133) was first dissolved in 1 phosphate buffer saline (PBS) (Fisher Scientific) solution (pH = 7.4) to prepare 32 mg mL 1 GOx solution. r;Y@Lo)&Fs47C0pt,*7rmq@1m.]H/fD>O9! 18 0 obj
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Glucose Oxidase from Aspergillus niger Type VII, lyophilized powder, 100,000 units/g solid (without added oxygen); CAS Number: 9001-37-0; EC Number: 232-601-0; Synonyms: G.Od.,-D-Glucose:oxygen 1-oxidoreductase,GOx; find Sigma-Aldrich-G2133 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich <442D676C75636F6E6F6C6163746F6E6520616E6420687964726F67656E207065726F7869646520706572206D696E20617420704820352E3120617420333520>Tj ET 0 g BT 515.00 342.05 Td <64656720432E>Tj ET 0 g BT 14.15 330.05 Td
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The following imaging buffer was added to the flow channel: 10 mM Tris, 50 mM NaCl, 0.1% Triton-X100, pH8.0 supplemented with 0.5 mg/ml Glucose Oxidase (Sigma, G2133), 40 ug/ml Catalase (Sigma, C100), 5% Glucose and 100 mM cysteamine (Sigma 30070). >>
Avidin (A9275), FITC-labeled avidin, Na2HPO4 (S0878), NaH2PO4 (S0751) and glucose oxidase (GOX, G2133, 180.2 U mg-1) were provided from Sigma. /Length 19 0 R
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Note that they also have a version 2 of this product, but we have honestly had less success with that product than with the original Lab-tek chambered coverglass. >>
Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. ]
Found inside Page 120To achieve the blinking process for dSTORM, the coverslips (thickness 0.17 mm) were incubated in a specific imaging buffer composed of 0.63 mg/ml glucose oxidase (G2133, Sigma-Aldrich) and its substrate (0.1 g/ml glucose), /Producer (SAP R/3 Release 620 )
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The following imaging buffer was added to the flow channel: 10 mM Tris, 50 mM NaCl, 0.1% Triton-X100, pH8.0 supplemented with 0.5 mg/ml Glucose Oxidase (Sigma, G2133), 40 ug/ml Catalase (Sigma, C100), 5% Glucose and 100 mM cysteamine (Sigma 30070). Glycerol 86%. endobj
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The buffer for glucose oxidase is a 0.1M sodium acetate buffer with a pH of 4. /Height 274
The reaction mixture consisted of 59.2 mM K-PIPES pH 6.8, 4.7 mM MgCl2, 3.2 mM potassium chloride, 2.6 mM potassium phosphate, 0.74 mM EGTA, 1.4 mM MgATP (Sigma A9187), 10% glycerol, 0.50 mg/mL pluronic F-127 (Sigma P2443), 0.22 mg/ml glucose oxidase (Sigma G2133), 3.2 mg/ml glucose, 0.038 mg/ml catalase (Sigma C40), 5.4 mM DTT, 2.0 mM Trolox . /Descent -270
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Although a more modest 1.5-fold improvement in H 2O 2 production was observed using a ve-enzyme cascade, due to H 2O Found inside Page 248Next mix solutions A and B and add 200 mg B - D - glucose , 40 mg ammonium chloride and 100-200 U of glucose oxidase ( Sigma type VII , Cat . No. G2133 ) . This is the final GDN reagent ; prepare fresh just before use . %PDF-1.5
M6500-25G) with pH 8.0, to provide a suitable chemical condition for . /Ascent 720
Tris pH 8, Ambion AM9856. Found inside Page 73Glucose Oxidase continued ADDITIONAL ACTIVITIES SUPPLIE CATALOG SPECIFIC UNITS DEFINITION PREPARATION FORM 100330 ICN 100331 Randox GO 833L Sigma G1262 Sigma G2133 > 200 U / mg 1 unit oxidizes 1 umol glucose / min at pH Lyophilized endstream
Fully revised and in its second edition, this standard reference on nano-optics is ideal for graduate students and researchers alike. To prepare glucose oxidase solutions for in vitro H 2 O 2 generation, it is recommended to obtain high purity glucose oxidase solid (e.g. 15. me$J0"GlF>i:-cu"S,jmipZlu!qP[Nrmq;Dme/F*r7;#Af`(a!"=3Tjf^o-p!S[,Ef+. !M3sA"R=G8:R/i
Resulting mixture was dilutedinto the final sample in order to achieve the following concentrations: 40 mM Glucose, 250 nM glucose oxidase, 60 nM catalase . >>
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Found inside Page 347 2001) PIPES (Sigma, P6757) Paclitaxel (Sigma, T7191) GTP (Jena Biosciences #NU-1012) GMPCPP (Jena Biosciences at 4 C Glucose (Sigma, G7528), 2 mg/ml in water and stored at 20 C Glucose oxidase (Sigma, G2133), 2 mg/ml in BRB80, <<
G2133), 1404 U/ml catalase (Sigma, Cat No. Cells and brain tissues were imaged in a photo-switching buffer comprising of 1% -mercaptoethanol (Sigma, #63689), and oxygen scavengers (glucose oxidase (Sigma, #G2133) and catalase (Sigma, #C40)) in 50mM Tris (Affymetrix, #22638100) + 10mM NaCl (Sigma, #S7653) + 10% glucose (Sigma, #G8270) at pH 8.0. It is convenient to make up stock solutions in BRB80 at 2M, 2 mg/ml, and 0.8 Found inside Page 84Reagents and resourcescont'd Reagent or resource Source Identifier Glucose Sigma G8270 Mercaptoethylamine (MEA) Fisher Scientific BP2664100 Glucose oxidase Sigma G2133 Catalase Sigma C3155 Critical commercial assays ThermoFisher C10637 <<
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mM Trolox (Sigma, 238813) and oxygen-scavenging enzymes, including 0.038 mg mL 1 catalase (Sigma, C40) and 0.22 mg mL 1 glucose oxidase (Sigma, G2133), fed with 3.3 mg mL 1 glucose (Sigma, G7528). <<
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G2133) . Glucose oxidase, Sigma G2133-10KU (Dissolve 37mg in 10mL of 50mM Sodium Acetate, pH ~5.5 (diluted from 3M, pH 5.5) for a 3.7mg/mL stock. Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. 4 0 obj
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Pretty much any vanilla widefield microscope will work for single molecule RNA FISH. 5mL 10x PBS, 5mL 37% formaldehyde (100% formalin), 40mL NF H2O (can leave in hood at RT for months). 1 0 obj
>>
Then the procedures were the same described above. >>
Glucose oxidase (Sigma G2133-50KU). 17. Molecular Weight null. Sigma-Aldrich G2133) Catalase (e.g. Glucose (24379) and CuCl 2 (23093) were purchased from Prolabo while EZ-link Sulfo-NHS-LC-biotin (21335) was acquired from Pierce. This new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in Microbial polysaccharides represent an attractive alternative to those from plants or macro algae. They can be produced from renewable sources including lignocellulosic waste streams. 15 0 obj
[Mangenot S, Hochrein M, Rdler J, Letellier L (2005) Curr Biol 15:430-435.] Found inside Page 316Assess concentration by UV absorbance (1 mg/ml = 1 A280), if > 20 mg/ml, then dilute, aliquot, and store at 20C.) D-glucose (Sigma-Aldrich, Cat # G7528) Glucose oxidase (Sigma-Aldrich, Cat # G2133) Catalase (Sigma-Aldrich, 15. <>>>
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Glucose oxidase. of glucose oxidase solution. 3.7 mg/ml Glucose Oxidase (Sigma G2133-10KU), stored at -20C (Dissolve in RNA-FREE sodium acetate in PH 5.2, Amresco 50mM (stock is 3.0M, catalog # E521-100ML) or Ambion, put in 37C for an hour until completely dissolved. <<
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Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. Formamide, Ambion AM9342 (Aliquot and store at 4C; bring to room temperature before making buffers. /Descent -270
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Prepare just before use. Found inside Page 141BP166-100) Catalase (Sigma-Aldrich, cat. no. G1345) Glucose oxidase (Sigma-Aldrich, cat. no. G2133) Trolox (Sigma-Aldrich, cat. no. 238813) - D -glucose (TCI, cat. no. G0047) Mouse anti-FLAG (Sigma-Aldrich, cat. no. 21 0 obj
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Glucose Oxidase, Aspergillus niger, Recombinant. /00017 17 0 R
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After the second wash, samples were placed in GLOX buffer (10 mM Tris-HCl, pH 7.5, 0.4% glucose, 2 SSC) at 4C until mounting. Glucose oxidase (Sigma, G2133), 2 mg/ml in BRB80, flash-frozen and stored at 20 C. /StemV 105
To reduce photobleaching, the slides were mounted in GLOX buffer (pH 8,10 mM, 2 SSC, 0.4% glucose), supplemented with 3.7 ng of glucose oxidase (Sigma G2133-10KU) and 1 l Catalase (Sigma 3515) prior to imaging. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. (glucose oxidase: G2133, peroxidase: P8250; Sigma Aldrich) and radioimmunoassay . Found inside Page 122Phosphate-buffered saline (PBS) STORM imaging buffer 50 mM Tris, pH 7.58.5, 10 mM NaCl, 0.5 mg/mL of glucose oxidase (G2133, Sigma-Aldrich), 40 g/mL of catalase (106810, Roche Applied Science), 10% (w/v) glucose, 14. endobj
Also, if you don't want to distinguish some of these dyes from each other (e.g. 7. endobj
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In particular, we will typically use the following dyes if we want to do 5 color FISH: Atto 488, Cy3, Cal Fluor 610/Alexa 594, Atto647N/Cy5, Atto 700. !in*]bukhbmng\]UWlMpARf*(6sn([F2f)kLCs8COWjQ"kMkh#^cr7BZns47YBht#g]n(6Rfmb%75f)smno'cV1"R"h9g@P6o)Y`%Fmf+Qij7:gchX]jar7<4Pj6@oQjQ@fgqptu/n*o!-rmq2*lMpARf*g6hiohZZfB;2bg@P?r
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Glucose Oxidase, Aspergillus niger, Recombinant Glucose Oxidase, Aspergillus niger, Recombinant, CAS 9001-37-0, oxidizes -D-glucose to D-glucono--lactone and H2O2 and converts D-glucono--lactone to gluconic acid. Stock is 1000 x concentrated in freezer; 143 mM of -mercaptoethanol (ME) at pH 8.0 to allow AF647 dye photoswitching OR /Subtype /Type1
Then add 1mL formamide and 1mL 20x SSC and volume up to 10mL (volume by eye in a 15mL Falcon tube is fine). Thus, monitoring the biocatalyzed generation of H 2 O 2 provides a versatile way for the development of bioassays. oxygen scavenging system and primary thiol: 50 mM tris, 10 mM NaCl, 10% w/v glucose, 168.8 U/ml glucose oxidase (Sigma, Cat No. By using this site, you agree to its use of cookies. endobj
You want to have a good light source, a good camera, and a good objective. 0.33 mg/mL glucose oxidase (G2133, Sigma Chemical Company, St. Louis, MO) Add 82.5 mg glucose oxidase to the 250 mL solution of 0.1 M potassium phosphate buffer containing glucose and 1 mM EDTA) 1400 U/mL catalase (C30, Sigma-Aldrich Chemical Company, St. Louis, MO) Add 1400 U of catalase per mL of 0.1 M potassium phosphate buffer containing 1 . Add 1g dextran sulfate to 7mL NF H2O and mix by rotation for a while (could take several minutes to a half an hour). 0000002982 00000 n
This site uses cookies from Google to deliver its services and to analyze traffic. Glucose transport and entry into cells is blocked by glucose-free medium or by 2-deoxyglucose. Peroxidase from horseradish (31941) was obtained from SERVA (Heidelberg, Germany). 14.15 554.45 Td <434153204E756D6265723A>Tj ET 0 g BT 172.55 554.45 Td <393030312D33372D30>Tj ET 0 g BT 14.15 542.45 Td <4D444C3A>Tj ET 0 g BT 172.55 542.45 Td <4D4643443030313331313832>Tj ET 0 g BT 14.15 506.45 Td
0.15 unit) was typically spotted onto the nitrocellulose. IV. /FontBBox [-177 -269 1123 866]
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Make aliquots and store at 4C away from light. Glucose Oxidase (Sigma G2133-10KU) (-20C freezer in dry box) Glycerol .
This solution should be prepared fresh and used immediately. 16 0 obj
A stock so-lution of 2 mg/mL GOx was prepared in 10 mM Tris at pH 7.4. In a Class 2 biological safety cabinet, dissolve solid GOX in tissue s4??"f?aN`fD=7RgSNNohW4+Vg]-15kh+8^f*LTso'"j*f@]fsf*3MmfCmt^m/5HMf`'Llj8@L0o@Wum#NRYlr7LStoDdDGf^m_m!;ulK!Ts4?i$s478VlMoH;qZ-Qof*(%$f*CmHf)u?Lf@]`qf)Yl-s47B!o[a*8q:>WPk5X$6i:-cu":+Mo!42^W$fCO,qZ-ZJo&A9Rlg7Xj66'nfB_GRfBheSqZ*u&f*C7)qUkf!nDjQ#!SH`1f*&GGg>r&-f)b@#s47;C!<1RQo&&'j!<1RNo&'Dt!JgaTf*(U&f8'G+f*(U&f8'G+f)bC#r7:u%oDdDFg@NqKkl968hXgd!"PE=tihcY+!K!42^W!T3JGf)YmDrRV,J!;ulK$f^L(#P-L!o@Nm?ZiAVXht$`t!V=tDf)tL%fC&[s"kW5+f@]`qf)ah$s47;(kl967Uj6tUpf`0Rl1A_(Ks47;2n,1c-be"oIN9rpp0cf)kgLq#/eFg[>*n"6SLqZiAV[fSJ/N!<1RTi:-NopXeHprmq>Ok5kSSf_Of_YX""mG+#rn.)If)b*trRV,N!;lfJs5sL9rrDrrfDk"9fDbjJs4@;6r7:rMoDdDFf^dYJoDdDCiq!-$"PWJ!j7*$! This volume presents current advanced technologies and methods used in super-resolution microscopy. 14. The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to Here's some info. Found inside Page 2403The enzymatic reaction of glucose to gluconic acid reduces the pH in the polymer microenvironment . Glucose oxidase ( G - 2133 , Sigma ) was incorporated into the polymer by coupling it to Sepharose beads using cyanogen bromide ( 18 ) "0fBD,_HJAAL:Zp@-,lH9XO!gEmf*C=(qZ-ZJo&Kc%!=.fff)adurmq2'oRYVFf7DP)f;7("o%O)p!UA\Ef)kXGjSd[7hXC?or:;35f*C7'nc0rf,i&FO!SI#Df)ah#rmq2)nq#DDf7DP)f;7("oDnmB!TZ$9f)t*lg]%3(#4L@8jO`tTrmq83n+6VWf)aUps47;2n,Co>
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